這個頁面上的內容需要較新版本的 Adobe Flash Player。

取得 Adobe Flash Player


謝道時
謝道時 Hsieh, Tao-shih

化學生物

中央研究院細胞與個體生物學研究所所長室

電話:(02)2789-9501

hsieh002@gate.sinica.edu.tw


研究興趣

  • 利用單分子螢光共振能量轉移技術分析 DNA 在細胞核內的移動情形
  • 研究拓撲異構酶參與在 DNA 鏈接與斷裂之機制
  • 研究 DNA 螺旋酶參與在穩定基因組與人類疾病之功能性探討
  • 篩選藉由抑制 DNA 拓撲異構酶/螺旋酶之抗癌藥物

榮譽

  • 2012 中央研究院第 29 屆院士
  • 2013 世界科學院第 24 屆院士

簡歷

1970 年國立臺灣大學學士; 1977 年加州大學柏克萊分校生物物理化學博士; 1977-1980 年史丹佛大學醫學中心博士後研究; 1981-1986 年美國杜克醫學中心生物化學系助理教授; 1986-1993 年美國杜克醫學中心生物化學系副教授; 1993 年起美國杜克醫學中心生物化學系教授; 2009 年起中央研究院細胞與個體生物學特聘研究員兼任所長


研究圖片

謝道時

代表著作

  1. Chen, S. H.; Plank J. L.; Wilcox S; Griffith J. D.; Hsieh T.-s.* “Top3α is Required During the Convergent Migration Step of Double Holliday Junction Dissolution” PLoS ONE, 2014, 9(1):e83582.
  2. Shun-Hsiao Lee; Grace Ee-Lu Siaw; Smaranda Willcox; Jack D. Griffith; Tao-shih Hsieh. “Synthesis and Dissolution of Hemicatenanes by Type IA DNA Topoisomerases” Proc. Natl. Acad. Sci. USA. 2013, 110(38), E3587–3594.
  3. Chen, S. H.; Chan, N.-L.; Hsieh T.-s. “New Mechanistic and Functional Insights into DNA Topoisomerases” Annual Review of Biochem. 2013, 82, 139–170.
  4. Chen, S. H.; Plank J. L.; Willcox S.; Griffith J. D.; Hsieh T.-s. “Improved Methods for Creating Migratable Holliday Junction Substrates” Nucleic Acids Research. 2012, 41(5):e60.
  5. Hohl A. M.; Thompson M.; Soshnev A. A.; Wu J.; Morris J.; Hsieh T.-s.; Wu C.-t.; Geyer P. K.; “Restoration of Topoisomerase 2 Function by Complementation of Defective Monomers in Drosophila” Genetics. 2012, 192, 843–856.
  6. Chen, Y.-t.; Collins, T.-R.; Guan Z.; Chen V. B.; Hsieh T.-s. “Probing Conformational Changes in Human DNA Topoisomerase IIα by Pulsed Alkylation Mass Spectrometry” J Biol Chem. 2012, 287, 25660–25668.
  7. Huang J.; Yu P.; Yuan C. T.; Ko H. C.; Tang J.; Hsieh T.-s. “Single-Particle Studies of the Plasmonic Fluorescence in Gold Nanocubes” Journal of Nanophotonics. 2012, 6, 0695021–0695026
  8. Chen, S. H.; Wu, C.-H.; Plank, J.-L.; Hsieh, T.-s. “Essential Functions of C Terminus of Drosophila Topoisomerase IIIα in Double Holliday Junction Dissolution” J Biol Chem. 2012, 287, 19346–19353.
  9. Chen, S. H.; Wu, J.; Hsieh, T.-s.* “Essential Functions of Topoisomerase IIIa in the Nucleus and Mitochondria” DNA Topoisomerases and Cancer, Ed. by Y. Pommier, Springer, 2012, 103–117.
  10. Fasulo B.; Koyama C.; Yu K. R.; Homola E. M.; Hsieh, T.-s.* Campbell S. D., Sullivan W. “Chk1 and Wee1 Kinases Coordinate DNA Replication, Chromosome Condensation and Anaphase Entry” Mol Biol Cell. 2012, 23, 1047–1057
  11. Lin, R.-K.; Zhou, N.; Lyu, Y. L.; Tsai, Y.-C.; Lu, C.-H.; Kerrigan, J.; Chen, Y.-t.; Guan, Z.; Hsieh, T.-s.; Liu, L. F. “Dietary Isothiocyanates-Induced Apoptosis: Involvement of Thiol Modification of Topoisomerase IIa” J Biol Chem. 2011, 286, 33591–33600.
  12. Capp, C.; Qian, Y.; Sage, H.; Huber, H.; Hsieh, T.-s.* “Separate and Combined Biochemical Activities of the Subunits of a Naturally Split Reverse Gyrase” J. Biol. Chem. 2010, 285, 39637–39645.
  13. Wu, J.; Feng, L.; Hsieh, T.-s.* “Totoisomerase 3α is Required for the Maintenance of Mitochondrial Genome and Male Germline Stem Cells in Drosophila” Proc. Natl. Acad. Sci. U.S.A. 2010, 107, 6228–6233.
  14. The phosphoCTD-interacting domain of Topoisomerase I. Wu, J., Phatnani, H.P., Hsieh, T.-s.; Greenleaf, A.L. Biochem Biophys Res Commun. (2010) Jun 18; 397(1):117-9. Epub 2010 May 20.
  15. Capp, C.; Wu, J.; Hsieh, T.-s.* “RecQ4: the Second Replicative Helicase?” Crit. Rev. Biochem. Mol. Biol. 2010, 45, 233–242.
  16. Capp, C.; Wu, J.; Hsieh, T.-s.* “Drosophila RecQ4 Has a 3'- 5'DNA Helicase Activity That is Essential for Viability” J. Biol. Chem. 2009, 284, 30845–30852.
  17. Plank, J. L.; Hsieh, T.-s.* “Helicase-Appended Topoisomerases: New Insight into the Mechanism of Directional Strand-Transfer” J. Biol. Chem. 2009, 284, 30737–30741 (JBC Mini-Review).
  18. Collins, T. R.; Hammes, G. G.; Hsieh, T-s.* “Analysis of the Eukaryotic Topoisomerase II DNA Gate: A Single-Molecule FRET and Structural Perspective” Nucleic Acids Res. 2009, 37, 712–720.
  19. Collins, T. R.; Hsieh, T.-s.* “Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer” Methods in Mol. Biol. 2009, 582, 59–70.
  20. Wu, J. H.; Capp, C.; Feng, L.; Hsieh, T.-s.* “Drosophila Homologues of Rothmund-Thomson Syndrome Gene: Essential Function in DNA Replication During Development” Dev. Biol. 2008, 323, 130–142. (Cover Picture)
  21. Desai, S. D.; Wood, L. J.; Hsieh, T.-s.; Marks, J. R.; Scott, G. L.; Giovanella, B. C.; Liu, L. F; “ISG15 as a Biomarker for Tumor Cell Sensitivity/Resistance to Topoisomerase I-Targeting Drugs.” Mol. Cancer Therapeutics 2008, 7, 1430–1439.
  22. Albertson, R.; Cao, J.; Hsieh, T.-s.; Sullivan, W. “Vesicles and Actin are Targeted to the Cleavage Furrow via Furrow Microtubules and the Central Spindle” J. Cell Biol. 2008, 181, 777–790.
  23. Webb, M. R.; Plank, J. L.; Long, D. T.; Hsieh, T.-s.; Kreuzer, K. N. “The Phage T4 Protein UvsW Drives Holliday Junction Branch Migration” J. Biol. Chem. 2007, 282, 34401–34411.
  24. Smiley, R. D.; Collins, T. R. L.; Hammes, G.; Hsieh, T.-s.*“Single Molecule Measurements of the Opening and Closing of the DNA Gate by Eukaryotic Type II Topoisomerase” Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 4840–4845.