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Department of Chemistry


LI, Hung-Wen

LI, Hung-Wen
Room B479, Chemistry Building
Fax: +886-2-3366-8671
Research Interest

  • Single-molecule biophysical chemistry.

  • DNA homologous recombination mechanism.

  • Protein-DNA interactions.

  • Optical microscopy tools.

Career and Service Experiences

  • B.S., 1993, National Taiwan University.

  • Ph.D., 2000, University of California at Berkeley.

  • Postdoctoral Research Fellow, 2000-2004, Brandeis University.

  • Assistant Professor, 2004-2007, McGill University.

  • Associate Professor, 2007-2014, National Taiwan University.

  • Professor, 2014-present, National Taiwan University.

Honors and Awards

  • Damon Runyon Cancer Research Fund Postdoctoral 
    Fellowship, 2000-2004.

  • Canada FQRNT New Investigator, 2005-2007.Young Investigator Award, Foundation for the Advancement of Outstanding Scholarship, 2007-2012.

  • Excellence in Teaching Award, National Taiwan University, 2010-2012.

  • Distinguished Teaching Award, National Taiwan University, 2013.

  • Outstanding Research Award, Ministry of Science and Technology, 2022.

Research Content
Selected Publications

  1. Lee, W., Iwasaki, H., Tsubouchi, H.* & Li, H.-W.*, “Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms”, Nucleic Acids Res., 51, 8550-62 (2023).

  2. Guh, C.L., Lei, K.H., Chen, Y.A., Jiang, Y.Z., Chang, H.Y., Liaw, H., Li, H.-W., Yen, H.Y. & Chi, P, “RAD51 paralogs synergize with RAD51 to protect reversed forks from cellular nucleases”, Nucleic Acids Res., (2023).

  3. Lei, K.-H., Yang, H.-L., Chang, H.-Y., Yeh, H.-Y., Nguyen, D. D., Lee, T.-Y., Lyu, X., Chastain, M., Chai, W., Li, H.-W.* & Chi, P.*, “Crosstalk between CST and RPA regulates RAD51 activity during replication stress” Nature Comm., 12, 6412 (2021).

  4. Lin, Y.-Y., Li, M.-H., Chang, Y.-C., Fu, P.-Y., Ohniwa, R. L., Li, H.-W.* & Lin, J.-J.*, “Dynamic DNA Shortening by Telomere-Binding Protein Cdc13” J. Am. Chem. Soc., 143, 5815-5825 (2021).

  5. Li, W.-C., Lee, C.-Y., Lan, W.-H., Woo, T.-T., Chen, C.-Y., Yeh, H.-Y., Chang, H.-Y., Liu, H.-C., Chen, C.-L., Chuang, C.-N., Hsueh, Y.-P., Li, H.-W.*, Chi, P.* & Wang, T.-F.*, “Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences” Proc. Natl. Acad. Sci. U.S.A., 118, e2007192118 (2021).

  6. Luo, S.-C., Yeh, H.-Y., Lan, W.-H., Wu, Y.-M., Yang, C.-H., Chang, H.-Y., Su, G.-C., Lee, C.-Y., Wu, W.-J., Li, H.-W., Ho, M.-C.*, Chi, P.*& Tsai, M.-D.* “Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures” Nature Comm., 12, 115 (2021).

  7. Lee, T.-Y., Li, Y.-C., Lin, M.-G., Hsiao, C.-D.* & Li, H.-W.*, "Single-Molecule Binding Characterization of Primosomal Protein PriA Involved in Replication Restart", Phys. Chem. Chem. Phys., 23, 13745-13751 (2021). 

  8. Lan, W.-H., Lin, S.-Y., Kao, C.-Y., Chang, W.-H., Yeh, H.-Y., Chang, H.-Y., Chi, P.* & Li, H.-W.* "Rad51 Facilitates Filament Assembly of Meiosis-Specific Dmc1 Recombinase" Proc. Natl. Acad. Sci. U.S.A., 117, 11257-11264 (2020).

  9. Chang, H.-Y., Lee, C.-Y., Lu, C.-H., Lee, W., Hang, H.-L., Yeh, H.-Y., Li, H.-W.* & Chi, P.* "Microcephaly family protein MCPH1 stabilizes RAD51 filaments" Nucleic Acids Res., 48, 9135-9146 (2020).

  10. Lin, Y.-H., Chu, C.-C., Wang, P.-Y., Fan, H.-F. Cox, M. M.*, & Li, H.-W.*, “A 5'-to-3' Strand Exchange Polarity is Intrinsic to RecA Nucleoprotein Filaments in the Absence of ATP Hydrolysis”, Nucleic Acids Res., 47, 5126-40 (2019).

  11. Lu, C.-H., Yeh, H.-Y., Su, G.-C., Ito, K., Kurokawa, Y., Iwasaki, H.*, Chi, P.* & Li, H.-W.* “Swi5-Sfr1 Stimulates Rad51 Recombinase Filament Assembly by Modulating Rad51 Dissociation”, Proc. Natl. Acad. Sci. U.S.A., 115, E10059-68 (2018).

  12. Wu, H.-Y., Lu, C.-H. & Li, H.-W.* “RecA-SSB Interaction Modulates RecA Nucleoprotein Filament Formation on SSB-Wrapped DNA”, Sci. Rep., 7, 11876 (2017).

  13. Li, J.-R., Yu, T.-Y., Chieh, I-.C., Lu, C.-Y., Lin, J.-J.* & Li, H.-W.*, “Pif1 Helicase Regulates Telomerase Activity by Removing from Telomere Ends”, Nucleic Acids Res., 42, 8527-8536 (2014).

  14. Chung, C. & Li, H.-W.*, “Direct Observation of RecBCD Helicase as Single-Stranded DNA Translocases” J. Am. Chem. Soc., 135, 8920-8925 (2013).

Update: 2023-11-10