國立臺灣大學化學系

• 單分子生物物理化學


• DNA 同源重組機制


• 蛋白質與DNA間的交互作用


• 光學顯微鏡技術

1993 年國立臺灣大學化學系學士；2000 年美國加州大學柏克萊校區化學博士；2000-2004 年美國 Brandeis 大學生化系博士後研究；2004-2007 年加拿大 McGill 大學化學系助理教授；2007-2014 年國立臺灣大學副教授；2014 年迄今國立臺灣大學教授；2018 年美國麻省理工學院生物系訪問教授

• 2000-2004 年Damon Runyon 癌症研究基金會博士後獎助


• 2005-2007 年加拿大FQRNT優秀年輕學者


• 2007-2012 年傑出人才發展基金會優秀年輕學者


• 2010-2012 年臺灣大學教學優良獎


• 2013 年臺灣大學教學傑出獎


• 2022 年科技部傑出研究獎

1. Wei, C.-D., Chang, H.-Y., Lu, C.-H., Chang, C.-C., Furukohri, A., Mwaniki, S., Shinohara, A., Chi, P.* & Li, H.-W.*, "Mei5-Sae3 stabilizes Dmc1 nucleating clusters for efficient Dmc1 assembly on RPA-coated single-stranded DNA", Nucleic Acids Res., 52, 11768 (2024).


2. Peng, J.-C., Chang, H.-Y., Sun, Y. L., Prentiss M., Li, H.-W. & Chi, P.*," Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination", Nature Comm., 15, 9266 (2024).


3. Lee, W., Iwasaki, H., Tsubouchi, H.* & Li, H.-W.*, "Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms", Nucleic Acids Res., 51, 8550 (2023).


4. Guh, C.L., Lei, K.H., Chen, Y.A., Jiang, Y.Z., Chang, H.Y., Liaw, H., Li, H.-W., Yen, H.Y. & Chi, P*, "RAD51 paralogs synergize with RAD51 to protect reversed forks from cellular nucleases", Nucleic Acids Res., 51, 11717 (2023).


5. Lei, K.-H., Yang, H.-L., Chang, H.-Y., Yeh, H.-Y., Nguyen, D. D., Lee, T.-Y., Lyu, X., Chastain, M., Chai, W., Li, H.-W.* & Chi, P.*, "Crosstalk between CST and RPA regulates RAD51 activity during replication stress", Nature Comm., 12, 6412 (2021).


6. Lin, Y.-Y., Li, M.-H., Chang, Y.-C., Fu, P.-Y., Ohniwa, R. L., Li, H.-W.* & Lin, J.-J.*, "Dynamic DNA Shortening by Telomere-Binding Protein Cdc13", J. Am. Chem. Soc., 143, 5815 (2021).


7. Li, W.-C., Lee, C.-Y., Lan, W.-H., Woo, T.-T., Chen, C.-Y., Yeh, H.-Y., Chang, H.-Y., Liu, H.-C., Chen, C.-L., Chuang, C.-N., Hsueh, Y.-P., Li, H.-W.*, Chi, P.* & Wang, T.-F.*, "Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences", Proc. Natl. Acad. Sci. U.S.A., 118, e2007192118 (2021).


8. Luo, S.-C., Yeh, H.-Y., Lan, W.-H., Wu, Y.-M., Yang, C.-H., Chang, H.-Y., Su, G.-C., Lee, C.-Y., Wu, W.-J., Li, H.-W., Ho, M.-C.*, Chi, P.*& Tsai, M.-D.*, "Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures", Nature Comm., 12, 115 (2021).


9. Lan, W.-H., Lin, S.-Y., Kao, C.-Y., Chang, W.-H., Yeh, H.-Y., Chang, H.-Y., Chi, P.* & Li, H.-W.* "Rad51 Facilitates Filament Assembly of Meiosis-Specific Dmc1 Recombinase", Proc. Natl. Acad. Sci. U.S.A., 117, 11257 (2020).


10. Chang, H.-Y., Lee, C.-Y., Lu, C.-H., Lee, W., Hang, H.-L., Yeh, H.-Y., Li, H.-W.* & Chi, P.* "Microcephaly family protein MCPH1 stabilizes RAD51 filaments", Nucleic Acids Res., 48, 9135 (2020).


11. Lin, Y.-H., Chu, C.-C., Wang, P.-Y., Fan, H.-F. Cox, M. M.*, & Li, H.-W.*, "A 5'-to-3' Strand Exchange Polarity is Intrinsic to RecA Nucleoprotein Filaments in the Absence of ATP Hydrolysis", Nucleic Acids Res., 47, 5126 (2019).


12. Lu, C.-H., Yeh, H.-Y., Su, G.-C., Ito, K., Kurokawa, Y., Iwasaki, H.*, Chi, P.* & Li, H.-W.* "Swi5-Sfr1 Stimulates Rad51 Recombinase Filament Assembly by Modulating Rad51 Dissociation", Proc. Natl. Acad. Sci. U.S.A., 115, E10059 (2018).


13. Li, J.-R., Yu, T.-Y., Chieh, I-.C., Lu, C.-Y., Lin, J.-J.* & Li, H.-W.*, "Pif1 Helicase Regulates Telomerase Activity by Removing from Telomere Ends", Nucleic Acids Res., 42, 8527 (2014).


14. Chung, C. & Li, H.-W.*, "Direct Observation of RecBCD Helicase as Single-Stranded DNA Translocases", J. Am. Chem. Soc., 135, 8920 (2013).